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NCJ Number: NCJ 199069     Find in a Library
Title: Automated STR Analysis for DNA Databases
Series: NIJ Research Report
Author(s): Mark W. Perlin Ph.D.
Corporate Author: Cybergenetics
United States of America
Date Published: 12/2002
Page Count: 20
Sponsoring Agency: National Institute of Justice
US Department of Justice
Office of Justice Programs
United States of America
Grant Number: 2000-IJ-CX-K005
Sale Source: NCJRS Photocopy Services
Box 6000
Rockville, MD 20849-6000
United States of America

Cybergenetics
160 North Craig Street, Suite 210
Pittsburgh, PA 15213
United States of America
Document: PDF 
Publisher: http://perlin@cybgen.com 
Type: Report (Study/Research)
Language: English
Country: United States of America
Annotation: This document focuses on the validation of automated short tandem repeat (STR) analyses of DNA databases.
Abstract: STR technology has enabled the rapid generation of DNA data for use in human identification. These data must be carefully analyzed. There is now an acute shortage of skilled data reviewers. The original STR data are carefully reviewed by two or more people to minimize error. To alleviate the critical labor shortage, the TrueAllele expert system was developed. The computer program automates virtually every human review function and provides consistent quality assessment and allele designation. The TrueAllele process begins with automated input of the data. The second step is automated gel image or capillary signal analysis. Step three is the allelic analysis, where the computer begins by analyzing the allelic ladders, transforming the data into DNA length coordinates and then accurately quantitating every DNA event in the data. In the final output step, TrueAllele automatically exports the data in format suitable for automated database import. A concordance validation study was conducted of the TrueAllele expert system for DNA database applications. Over 48,000 genotypes were processed using diverse STR panels and automated DNA sequences. It was demonstrated that the automated analysis and quality assurance system was accurate and that it could reduce the human time, error, effort, and costs associated with conventional labor-intensive review of Combined DNA Index System (CODIS) data. The automated methods were objective and comprehensive, providing for more uniform application of DNA forensic technology. 4 figures, 4 tables, 9 references
Main Term(s): Testing and measurement ; DNA fingerprinting
Index Term(s): Crime Laboratories (Crime Labs) ; Trace evidence ; Suspect identification ; Blood/body fluid analysis ; Hair and fiber analysis ; Forensics/Forensic Sciences ; NIJ grant-related documents
   
  To cite this abstract, use the following link:
https://www.ncjrs.gov/App/Publications/abstract.aspx?ID=199069

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