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NCJ Number: NCJ 213893     Find in a Library
Title: dHPLC Method for Forensic DNA Analysis
Author(s): Patrick S. Callery ; Bernard Boswell ; Peter M. Gannett ; Robert L. Haining ; Madhu Sanga ; Padma Tirumalai ; Timothy S. Tracy
Date Published: 2005
Page Count: 17
Sponsoring Agency: National Institute of Justice
US Department of Justice
Office of Justice Programs
United States of America
Grant Number: 2000-DN-VX-K001
Sale Source: National Institute of Justice/NCJRS
Box 6000
Rockville, MD 20849
United States of America
Document: PDF 
Type: Test/Measurement
Language: English
Country: United States of America
Annotation: This exploratory study investigated the usefulness of denaturing high-performance liquid chromatography (dHPLC) as an analytic method for DNA testing.
Abstract: Overall, the results indicate that Single Nucleotide Polymorphism (SNP) analysis by dHPLC is a useful screen of DNA samples prior to sequencing. Additionally, dHPLC was found to be an effective method for separating adducts formed from the parent oligonucleotide, indicating that dHPLC may provide a powerful forensic tool, especially when coupled with mass spectrometry. Strengths of the dHPLC analysis method are ease of instrument operation, shorter sample analysis time, and potential for the development of new analysis methods. The primary weakness of dHPLC as a DNA analysis method is the absence of robust methods that can be routinely applied by crime laboratories. Research methods involved the extraction of DNA from hair shaft material from 35 individuals using the Promega Tissue and Hair Extraction kit together with DNA IQ. Future research should focus on the role of dHPLC technology and mass spectrometry in the development of forensic biomarkers. References, figures
Main Term(s): Forensics/Forensic Sciences ; DNA fingerprinting
Index Term(s): Crime Laboratories (Crime Labs) ; NIJ grant-related documents
   
  To cite this abstract, use the following link:
https://www.ncjrs.gov/App/Publications/abstract.aspx?ID=235397

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