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NCJ Number: NCJ 215339     Find in a Library
Title: Development of a High-Throughput Method to Isolate Sperm DNA in Sexual Assault Cases
Author(s): Carll Ladd Ph.D. ; Eric J. Carita M.S. ; Elaine M. Pagliaro J.D. ; Alex Garvin Ph.D. ; Andrew R. Crumbie J.D. ; Henry C. Lee Ph.D.
Date Published: 2006
Page Count: 31
Sponsoring Agency: National Institute of Justice
US Department of Justice
Office of Justice Programs
United States of America
Grant Number: 2003-IJ-CX-K013
Sale Source: National Institute of Justice/NCJRS
Box 6000
Rockville, MD 20849
United States of America

NCJRS Photocopy Services
Box 6000
Rockville, MD 20849-6000
United States of America
Document: PDF 
Type: Report (Study/Research)
Language: English
Country: United States of America
Annotation: This study evaluated and optimized the track-etch filtration method for isolating sperm for DNA analysis and compared this method to the standard differential extraction protocol using multiple amplification kits and sequencer platforms.
Abstract: Results indicated that the ISOPORE track-etch filter was effective at identifying the DNA profile of a semen donor from a sample of mixed body fluids. The track-etch filter separation technique was equal or superior to the standard differential extraction method for identifying DNA profiles, but the track-etch filter method took much less time than the standard differential extraction method, saving over 2 hours. The track-etch filter method was improved by minimizing the problem of filter clogging through RNase treatment. However, it will be necessary to remove epithelial cell debris without a pelleting step in order to fully automate the method. Automation is desirable to reduce the thousands of backlogged sexual assault cases waiting to be processed. Semen was collected from healthy male volunteers and an average number of sperm per microliter was determined through the use of a hemocytometer. Buccal swabs were collected from healthy female donors and approximately 100,000 sperm were added to the swabs and were then extracted using both the track-etch filtration method with and without RNase treatment and the standard differential extraction method. Additionally, 10 sexual assault evidentiary samples from 9 previously adjudicated cases were processed using both the track-etch filtration method and the standard differential extraction method. Simple fixes needed on the track-etch filtration method included filter leakage and popping. Tables, figures, references
Main Term(s): Comparative analysis ; Forensics/Forensic Sciences ; DNA fingerprinting
Index Term(s): Evidence identification and analysis ; Research methods ; Sexual assault ; NIJ final report ; NIJ grant-related documents
   
  To cite this abstract, use the following link:
https://www.ncjrs.gov/App/Publications/abstract.aspx?ID=236922

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