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NCJ Number: NCJ 217267     Find in a Library
Title: Development of Microdevice Solid-Phase Purification Utilizing Dual Pressure/Electro-Elution for Concentration and Enhanced Recovery of DNA
Author(s): Joan Bienvenue
Date Published: 02/2007
Page Count: 23
Sponsoring Agency: National Institute of Justice
US Department of Justice
Office of Justice Programs
United States of America
Grant Number: 2005-DA-BX-K100
Sale Source: National Institute of Justice/NCJRS
Box 6000
Rockville, MD 20849
United States of America

NCJRS Photocopy Services
Box 6000
Rockville, MD 20849-6000
United States of America
Document: PDF 
Type: Report (Study/Research) ; Test/Measurement
Language: English
Country: United States of America
Annotation: This paper describes the evaluation of a device that uses an electric field during the DNA elution stage of the solid phase extraction process in order to enhance recovery of DNA and provide a more concentrated sample for subsequent genetic analysis.
Abstract: The evaluation demonstrated the application of an electric field while retaining and capturing DNA with precision elution and maintaining solution flow through the device designed for this evaluation. This was the first example of electrophoretically retained DNA during the course of micro-solid phase purification. It is the first step toward addressing the volume incompatibility between the DNA purification and PCR amplification domains in integrated microfluidic devices. The paper advises, however, that further development of the device design and protocol is required in order to ensure that the majority of the eluting DNA is retained. The preliminary results of this evaluation suggest that with proper evaluation and continued design development, the complete capture, concentration, and retention of DNA can be achieved. This paper describes the preliminary design for a glass microdevice capable of electro-solid phase extraction and dual pressure/electro-elution. Using this device, a typical solid phase extraction (sample load, protein wash, and DNA elutions) was performed using pressure-driven flow. The electric field was imposed during the final elution step in order to trap DNA as it exited the device. 12 figures and 24 references
Main Term(s): Criminology
Index Term(s): Technology transfer ; Science and Technology ; Forensics/Forensic Sciences ; Investigative techniques ; DNA fingerprinting ; NIJ final report ; NIJ grant-related documents
   
  To cite this abstract, use the following link:
https://www.ncjrs.gov/App/Publications/abstract.aspx?ID=238894

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