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NCJ Number: NCJ 227497   Add to Shopping cart   Find in a Library
Title: Laser Microdissection as a Technique to Resolve Mixtures and Improve the Analysis of Difficult Evidence Samples
Author(s): Robert Driscoll M.F.S. ; Dane Plaza B.S. ; Robert Bever Ph.D.
Corporate Author: Bode Technology Group, Inc.
United States of America
Date Published: 10/2008
Page Count: 95
Sponsoring Agency: National Institute of Justice
US Department of Justice
Office of Justice Programs
United States of America
Grant Number: 2006-DN-BX-K032
Sale Source: National Institute of Justice/NCJRS
Box 6000
Rockville, MD 20849
United States of America

NCJRS Photocopy Services
Box 6000
Rockville, MD 20849-6000
United States of America
Document: PDF 
Type: Report (Study/Research)
Language: English
Country: United States of America
Annotation: The research reported here focused on the further investigation of the various aspects and benefits of laser microdissection (LM), which has proven to be an effective method for cell mixture separations in the forensic laboratory while improving the collection and DNA analysis associated with low copy number (LCN) samples.
Abstract: This work found that the swabbing of substrates followed by a cellulose-aided elution increased the number of cells recovered for LM slide preparation. The QIAamp Micro Extraction kit was found to be efficient and reliable for extracting low-level samples collected by LM. Amplification results indicate that the Applied Biosystems AmpFlSTR MiniFiler kit is the optimal choice for amplifying LCN samples collected with LM instruments. Additional research was also completed on the cellular separation of problematic sample mixtures. Although sperm and epithelial cell sexual-assault mixtures can easily be separated based upon morphological differences, combinations of the same cell type are more difficult to separate. For such problematic samples, a protocol was developed that is capable of successfully separating male/female cellular mixtures of similar morphology by using chromosome X/Y FISH (fluorescent in situ hybridization) probing. In the course of performing these LM evaluations, the Pix Cell and Zeiss PALM Microbeam instruments were assessed in order to determine optimal processing protocols. They proved to be suitable for LCN and mixture evidence processing. By applying the findings of this research, forensic labs can now successfully and efficiently resolve various LCN sample mixtures by incorporating FISH, LM, and optimized elution/extraction/amplification methods into their standard operating protocols. 26 figures, 13 tables, 25 references, and a listing of 6 publications that disseminate research findings
Main Term(s): Criminology
Index Term(s): Evidence identification and analysis ; Sexual assault ; Rape investigations ; Forensics/Forensic Sciences ; Investigative techniques ; DNA fingerprinting ; NIJ final report
   
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https://www.ncjrs.gov/App/Publications/abstract.aspx?ID=249501

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