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NCJ Number: NCJ 236826     Find in a Library
Title: Sample Collection System for DNA Analysis of Forensic Evidence: Towards Practical, Fully-Integrated STR Analysis
Author(s): Eugene Tan
Corporate Author: NetBio Inc.
United States of America
Date Published: 2011
Page Count: 45
Sponsoring Agency: National Institute of Justice
US Department of Justice
Office of Justice Programs
United States of America
Grant Number: 2008-DN-BX-K010
Sale Source: National Institute of Justice/NCJRS
Box 6000
Rockville, MD 20849
United States of America

NCJRS Photocopy Services
Box 6000
Rockville, MD 20849-6000
United States of America
Document: PDF 
Type: Report (Study/Research)
Language: English
Country: United States of America
Annotation: This research developed a sample collection system for law enforcement agents to use in collecting, protecting, and documenting biological evidence; and it performed initial processing steps in a format compatible with rapid sample-in to results-out microfluidic DNA analysis.
Abstract: The sample collection system developed consists of an evidence-collection device (a collecting swab and accompanying tube for swab insertion) and a sample processing cartridge. This “Smart Cartridge” lyses cells, makes soluble and concentrates DNA, and transfers the DNA solution to a microfluidic biochip. The microfluidic biochip will process the DNA solution to generate purified DNA compatible with the requirements for STR analysis. The evidence-collection device is designed to accept blood, buccal cells, saliva, and cellular samples. Cotton, modified cellulose, foam, nylon, polyester, and rayon-tipped swabs were evaluated for reproducibility of cell collection, ease of collection, tolerance to collection, protocol, liquid absorption, and use in forensic applications. The Bode SecurSwab was selected for the sample collection system. Operation of the system requires the user to collect a swab sample, insert the swab into the collection tube, place the tube into the Smart Cartridge, and press a start button; no further intervention is required. The DNA generated by the sample collection system will then be transferred automatically to NetBio’s fully integrated microfluidic biochip for STR typing, which will generate an STR profile in 45 minutes from sample introduction, with minimal operating requirements. Several approaches to the sample processing steps were evaluated, and those selected were integrated to develop an optimized Smart Cartridge. 25 figures and 13 references
Main Term(s): Police policies and procedures
Index Term(s): Evidence collection ; Victim identification ; Suspect identification ; Efficiency ; Productivity ; Investigative techniques ; DNA fingerprinting ; NIJ final report
   
  To cite this abstract, use the following link:
https://www.ncjrs.gov/App/Publications/abstract.aspx?ID=258846

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