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NCJ Number: NCJ 237838   Add to Shopping cart   Find in a Library
Title: Investigations on the Use of Samplematrix to Stabilize Crime Scene Biological Samples for Optimized Analysis and Room Temperature Storage
Author(s): Katherine A. Roberts ; Donald J. Johnson
Date Published: 02/2012
Page Count: 296
Sponsoring Agency: National Institute of Justice
US Department of Justice
Office of Justice Programs
United States of America
Grant Number: 2007-DN-BX-K172
Sale Source: National Institute of Justice/NCJRS
Box 6000
Rockville, MD 20849
United States of America

NCJRS Photocopy Services
Box 6000
Rockville, MD 20849-6000
United States of America
Document: PDF 
Type: Survey
Language: English
Country: United States of America
Annotation: This research evaluates a proprietary platform technology for the dry storage of biological materials at ambient temperature.
Abstract: This coating-agent study evaluated the total amount of DNA recovered from blood, saliva, and semen samples (n=7,500) deposited on a cotton swab following a 6-24 month storage period. The study found that when considering the experimental variables for the wetting and environmental insult studies, a comparison of the various storage conditions showed that SampleMatrix-protected samples provided mean DNA yields that were equivalent or greater compared with the unprotected controls. In addition, when comparing mean recovery as a function of substrate or dilution series, significant differences in the mean recovery were observed. The data also indicated that there was no disadvantage to storing samples at room temperature compared with frozen storage; however, statistical analyses were performed in order to assess the differences in the mean DNA yield for the protected and unprotected samples. Although the results indicated that the differences were not statistically significant, a larger number of replicates are required in order to determine the significance of the treatment methods. The samples were subjected to the following experimental variables: protection with one of two SampleMatrix formulations (SM1 and SM2) as a post-collection treatment of the sample, extraction method, storage medium, dilution factor, and the method used to apply SampleMatrix as a coating agent (wet versus dry samples). The wetting agent study also evaluated the performance of the two formulations of SampleMatrix compared to water in the collection and preservation of DNA recovered from blood, saliva, and semen samples. These samples were subjected to the following experimental variables: the extraction method, the storage medium, dilution factor, and the substrate used to deposit the samples prior to recovery with cotton swabs. Two environmental-insult studies were performed. 195 figures, 71 references, and a list of publications with research findings
Main Term(s): Police policies and procedures
Index Term(s): Evidence collection ; Evidence preservation ; Blood/body fluid analysis ; Evidence technicians ; NIJ final report
   
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https://www.ncjrs.gov/App/Publications/abstract.aspx?ID=259870

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