This was done by first extending the previously developed statistical methodology to allow for self-calibration in controlling rates of false non-matches and then empirically validating the methodology developed by performing experiments that used a different type of tool mark. The authors' previous work focused on the use of the Mann-Whitney U-statistic as an index for assessing the similarity of toolmarks. Although it has been empirically shown to be useful in sorting mark-pairs made by the same tool from mark-pairs made with different tools, it is also influenced by many other aspects of the tool mark structure, such that a single value cannot be used as objective evidence for or against a match. The current work overcame this difficulty by using multiple test marks made in the laboratory in a "self-calibrated" analysis. Comparison values between lab marks that are known to match form the basis for comparisons between lab marks and evidence marks. This eliminates the need for "universal" critical values (i.e., single sets of constant references value such as those found in commonly used statistical tables) for the comparison index. A formal statistical analysis based on likelihood functions has been developed to allow for control of false non-match calls. The methodology developed was empirically validated by performing experiments that used a different type of tool mark. Successful validation of the methodology has produced a wide range of possible future applications for the developed statistical algorithm that could revolutionize comparative tool mark analysis. 25 figures, 4 tables, 26 references, and listings in which research findings have been disseminated